2fnw
From Proteopedia
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Pseudomonas aeruginosa E2Q/H83Q/M109H-azurin RE(PHEN)(CO)3
Overview
The triplet metal-to-ligand charge transfer ((3)MLCT) dynamics of two, structurally characterized Re(I)(CO)(3)(phen)(HisX)-modified (phen =, 1,10-phenanthroline; X = 83, 109) Pseudomonas aeruginosa azurins have been, investigated by picosecond time-resolved infrared (TRIR) spectroscopy in, aqueous (D(2)O) solution. The (3)MLCT relaxation dynamics exhibited by the, two Re(I)-azurins are very different from those of the sensitizer, [Re(I)(CO)(3)(phen)(im)](+) (im = imidazole). Whereas the Re(I)(CO)(3), intramolecular vibrational relaxation in Re(I)(CO)(3)(phen)(HisX)Az (4 ps), is similar to that of [Re(I)(CO)(3)(phen)(im)](+) (2 ps), the medium, relaxation is much slower ( approximately 250 vs 9.5 ps); the 250-ps, relaxation is attributable to reorientation of D(2)O molecules as well as, structural reorganization of the rhenium chromophore and nearby polar, amino acids in each of the modified proteins.
About this Structure
2FNW is a Single protein structure of sequence from Pseudomonas aeruginosa with and as ligands. Full crystallographic information is available from OCA.
Reference
Excited-state dynamics of structurally characterized [ReI(CO)3(phen)(HisX)]+ (X = 83, 109) Pseudomonas aeruginosa azurins in aqueous solution., Blanco-Rodriguez AM, Busby M, Gradinaru C, Crane BR, Di Bilio AJ, Matousek P, Towrie M, Leigh BS, Richards JH, Vlcek A Jr, Gray HB, J Am Chem Soc. 2006 Apr 5;128(13):4365-70. PMID:16569013
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