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1wbp
From Proteopedia
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SRPK1 BOUND TO 9MER DOCKING MOTIF PEPTIDE
Overview
The arginine-serine (RS)-rich domain of the SR protein ASF/SF2 is, phosphorylated by SR protein kinases (SRPKs) and Clk/Sty kinases. However, the mode of phosphorylation by these kinases and their coordination in the, biological regulation of ASF/SF2 is unknown. Here, we report the crystal, structure of an active fragment of human SRPK1 bound to a peptide derived, from an SR protein. This structure led us to identify a docking motif in, ASF/SF2. We find that this docking motif restricts phosphorylation of, ASF/SF2 by SRPK1 to the N-terminal part of the RS domain - a property, essential for its assembly into nuclear speckles. We further show that, Clk/Sty causes release of ASF/SF2 from speckles by phosphorylating the, C-terminal part of its RS domain. These results suggest that the ... [(full description)]
About this Structure
1WBP is a [Protein complex] structure of sequences from [Homo sapiens] with ACT and ADP as [ligands]. Active as [Transferred entry: 2.7.11.1], with EC number [2.7.1.37]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Interplay between SRPK and Clk/Sty kinases in phosphorylation of the splicing factor ASF/SF2 is regulated by a docking motif in ASF/SF2., Ngo JC, Chakrabarti S, Ding JH, Velazquez-Dones A, Nolen B, Aubol BE, Adams JA, Fu XD, Ghosh G, Mol Cell. 2005 Oct 7;20(1):77-89. PMID:16209947
Page seeded by OCA on Tue Oct 30 12:13:30 2007
Categories: Homo sapiens | Protein complex | Transferred entry: 2.7.11.1 | Adams, J.A. | Aubol, B.E. | Chakrabarti, S. | Ding, J.H. | Fu, X.D. | Ghosh, G. | Ngo, J.C. | Nolen, B. | Velazquez-Dones, A. | ACT | ADP | Alternative splicing | Atp-binding | Chromosome partition | Differentiation | Kinase | Mrna processing | Mrna splicing | Nuclear protein | Nucleotide-binding | Phosphorylation | Serine/threonine-protein kinase | Srpk | Transferase
