Group:MUZIC:CARP
From Proteopedia
Introduction
CARDIAC ANKYRIN REPEAT PROTEIN; CARPbelongs to the conserved muscle ankyrin repeat protein (MARP) family, Also known as Ankrd1.MARP family consist of two more homologous members Ankyrin repeat domain containing protein 2 (Ankrd2) and Diabetes related ankyrin repeat protein DARP.
| |||||||||
| 1n0r, resolution 1.50Å () | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Ligands: | |||||||||
| Related: | 1n0q | ||||||||
| |||||||||
| |||||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Contents |
Structure
Using the N2A region of titin as bait in a yeast 2-hybrid screen of a skeletal muscle cDNA library cloned ANKRD1, which they called CARP. [1] full-length cDNA encoding ANKRD1 contains a nuclear localization signal, 4 tandem ankyrin-like repeats, multiple phosphorylation sites, and a PEST-rich region, which is typically found in rapidly degraded proteins.[2]
Gene Function
CARP plays a structural role by interacting with the Z disc protein titin. Ankrd1/CARP is a part of the titin-mechanosensory signaling complex in the sarcomere and in response to stretch it translocates to the nucleus where it participates in the regulation of cardiac genes as a transcriptional co-repressor.Ankrd1/CARP has a role in the propagation of signals initiated by myogenic regulatory factors (MRFs) during myogenesis.[3] CARP overexpression in muscle fibres fails to affect their calibre, indicating that CARP per se cannot initiate atrophy. However, a switch towards fast-twitch fibres is observed, suggesting that CARP plays a role in skeletal muscle plasticity.[4] Mutations in the ANKRD1 gene encoding CARP are responsible for human dilated cardiomyopathy.[5]
Localization
Ankrd1/CARP is a part of the titin-mechanosensory signaling complex in the sarcomere and in response to stretch it translocates to the nucleus where it participates in the regulation of cardiac genes as a transcriptional co-repressor.
CARP Interactions
CARP interacts with the titin in the Z disc.
