2oiu
From Proteopedia
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L1 Ribozyme Ligase circular adduct
Overview
Life originated, according to the RNA World hypothesis, from, self-replicating ribozymes that catalyzed ligation of RNA fragments. We, have solved the 2.6 angstrom crystal structure of a ligase ribozyme that, catalyzes regiospecific formation of a 5' to 3' phosphodiester bond, between the 5'-triphosphate and the 3'-hydroxyl termini of two RNA, fragments. Invariant residues form tertiary contacts that stabilize a, flexible stem of the ribozyme at the ligation site, where an essential, magnesium ion coordinates three phosphates. The structure of the active, site permits us to suggest how transition-state stabilization and a, general base may catalyze the ligation reaction required for prebiotic RNA, assembly.
About this Structure
2OIU is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
The structural basis of ribozyme-catalyzed RNA assembly., Robertson MP, Scott WG, Science. 2007 Mar 16;315(5818):1549-53. PMID:17363667
Page seeded by OCA on Tue Jan 29 21:11:38 2008
Categories: Protein complex | Robertson, M.P | Scott, W.G. | MG | Active site | In vitro selection | Ligase | Ribozyme | Rna
