6bna

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spinqualitylabelsall models 6bna, resolution 2.210Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

BINDING OF AN ANTITUMOR DRUG TO DNA. NETROPSIN AND C-G-C-G-A-A-T-T-BRC-G-C-G

Overview

The antitumor antibiotic netropsin has been co-crystallized with a, double-helical B-DNA dodecanucleotide of sequence:, C-G-C-G-A-A-T-T-BrC-G-C-G, and the structure of the complex has been, solved by X-ray diffraction at a resolution of 2.2 A. The structure has, been refined independently by Jack-Levitt and Hendrickson-Konnert, least-squares methods, leading to a final residual error of 0.257 by the, Jack-Levitt approach (0.211 for two-sigma data) or 0.248 by the, Hendrickson-Konnert approach, with no significant difference between, refined structures. The netropsin molecule displaces the spine of, hydration and fits snugly within the minor groove in the A-A-T-T center., It widens the groove slightly and bends the helix axis back by 8 degrees, but neither unwinds nor elongates the double helix. The drug molecule is, held in place by amide NH hydrogen bonds that bridge adenine N-3 and, thymine O-2 atoms, exactly as with the spine of hydration. The requirement, of A X T base-pairs in the binding site arises because the N-2 amino group, of guanine would demand impermissibly close contacts with netropsin. It is, proposed that substitution of imidazole for pyrrole in netropsin should, create a family of "lexitropsins" capable of reading G X C-containing base, sequences.

About this Structure

6BNA is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.

Reference

Binding of an antitumor drug to DNA, Netropsin and C-G-C-G-A-A-T-T-BrC-G-C-G., Kopka ML, Yoon C, Goodsell D, Pjura P, Dickerson RE, J Mol Biol. 1985 Jun 25;183(4):553-63. PMID:2991536

Page seeded by OCA on Tue Jan 29 21:49:38 2008