2j4r

From Proteopedia

Revision as of 08:57, 31 January 2008 by OCA (Talk | contribs)
Jump to: navigation, search
Image:2j4r.gif

2j4r, resolution 2.71Å

Drag the structure with the mouse to rotate

STRUCTURAL STUDY OF THE AQUIFEX AEOLICUS PPX-GPPA ENZYME

Overview

The crystal structure of the prototype exopolyphosphatase/guanosine, pentaphosphate phosphohydrolase protein family member from Aquifex, aeolicus in complex with the intracellular second messenger guanosine, tetraphosphate was determined at 2.7-A resolution. The hydrolytic base is, identified as E119. The dual specificity established for the Escherichia, coli homolog is shown to be compatible with a common active site for, guanosine pentaphosphate and polyphosphate hydrolysis. Distinct and, different degrees of closure between the two domains of the enzyme are, associated with substrate binding. The arginines R22 and R267, residing in, different domains, are crucial for guanosine pentaphosphate specificity as, they interact with the unique 3'-ribose phosphorylation.

About this Structure

2J4R is a Single protein structure of sequence from Aquifex aeolicus with as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.

Reference

Structure of the PPX/GPPA phosphatase from Aquifex aeolicus in complex with the alarmone ppGpp., Kristensen O, Ross B, Gajhede M, J Mol Biol. 2008 Feb 1;375(5):1469-76. Epub 2007 Dec 4. PMID:18155044

Page seeded by OCA on Thu Jan 31 10:57:22 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2j4r"
Personal tools