2owm

From Proteopedia

Motor domain of Neurospora crassa kinesin-3 (NcKin3)

Overview

Neurospora crassa kinesin NcKin3 belongs to a unique fungal-specific, subgroup of small Kinesin-3-related motor proteins. One of its functions, appears to be the transport of mitochondria along microtubules. Here, we, present the X-ray structure of a C-terminally truncated monomeric, construct of NcKin3 comprising the motor domain and the neck linker, and a, 3-D image reconstruction of this motor domain bound to microtubules, by, cryoelectron microscopy. The protein contains Mg.ADP bound to the active, site, yet the structure resembles an ATP-bound state. By comparison with, structures of the Kinesin-3 motor Kif1A in different nucleotide states, (Kikkawa, M. et al. (2001) Nature (London, U.K.) 411, 439-445), the NcKin3, structure corresponds to the AMPPCP complex of Kif1A rather than the, AMPPNP complex. NcKin3-specific differences in the coordination of the, nucleotide and asymmetric interactions between adjacent molecules in the, crystal are discussed in the context of the unusual kinetics of the, dimeric wild-type motor and the monomeric construct used for crystal, structure analysis. The NcKin3 motor decorates microtubules at a, stoichiometry of one head per alphabeta-tubulin heterodimer, thereby, forming an axial periodicity of 8 nm. In spite of unusual extensions at, the N-terminus and within flexible loops L2, L8a, and L12 (corresponding, to the K-loop of monomeric kinesins), the microtubule binding geometry is, similar to that of other members of the kinesin family.

About this Structure

2OWM is a Single protein structure of sequence from Neurospora crassa with and as ligands. Active as Plus-end-directed kinesin ATPase, with EC number 3.6.4.4 Known structural/functional Sites: , , , , , , and . Full crystallographic information is available from OCA.

Reference

X-ray Structure and Microtubule Interaction of the Motor Domain of Neurospora crassa NcKin3, a Kinesin with Unusual Processivity(,)., Marx A, Muller J, Mandelkow EM, Woehlke G, Bouchet-Marquis C, Hoenger A, Mandelkow E, Biochemistry. 2008 Jan 19;. PMID:18205396

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