1pfo

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spinqualitylabelsall models 1pfo, resolution 2.2Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

PERFRINGOLYSIN O

Overview

The mechanisms by which proteins gain entry into membranes is a, fundamental problem in biology. Here, we present the first crystal, structure of a thiol-activated cytolysin, perfringolysin O, a member of a, large family of toxins that kill eukaryotic cells by punching holes in, their membranes. The molecule adopts an unusually elongated shape rich in, beta sheet. We have used electron microscopy data to construct a detailed, model of the membrane channel form of the toxin. The structures reveal a, novel mechanism for membrane insertion. Surprisingly, the toxin receptor, cholesterol, appears to play multiple roles: targeting, promotion of, oligomerization, triggering a membrane insertion competent form, and, stabilizing the membrane pore.

About this Structure

1PFO is a [Single protein] structure of sequence from [Clostridium perfringens]. Full crystallographic information is available from [OCA].

Reference

Structure of a cholesterol-binding, thiol-activated cytolysin and a model of its membrane form., Rossjohn J, Feil SC, McKinstry WJ, Tweten RK, Parker MW, Cell. 1997 May 30;89(5):685-92. PMID:9182756

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