1h6l

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spinqualitylabelsall models 1h6l, resolution 1.8Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

BETA-PROPELLER PHYTASE IN COMPLEX WITH PHOSPHATE AND CALCIUM IONS

Overview

BACKGROUND: Phytases hydrolyze phytic acid (myo-inositol-hexakisphosphate), to less-phosphorylated myo-inositol derivatives and inorganic phosphate., Phytases are used in animal feed to reduce phosphate pollution in the, environment. Recently, a thermostable, calcium-dependent Bacillus phytase, was identified that represents the first example of the beta propeller, fold exhibiting phosphatase activity. We sought to delineate the catalytic, mechanism and property of this enzyme. RESULTS: The crystal structure of, the enzyme in complex with inorganic phosphate reveals that two phosphates, and four calcium ions are tightly bound at the active site. Mutation of, the residues involved in the calcium chelation results in severe defects, in the enzyme's activity. One phosphate ion, chelating all of the four, calcium ions, is close to a water molecule bridging two of the bound, calcium ions. Fluoride ion, which is expected to replace this water, molecule, is an uncompetitive inhibitor of the enzyme. The enzyme is able, to hydrolyze any of the six phosphate groups of phytate. CONCLUSIONS: The, enzyme reaction is likely to proceed through a direct attack of the, metal-bridging water molecule on the phosphorous atom of a substrate and, the subsequent stabilization of the pentavalent transition state by the, bound calcium ions. The enzyme has two phosphate binding sites, the, "cleavage site", which is responsible for the hydrolysis of a substrate, and the "affinity site", which increases the binding affinity for, substrates containing adjacent phosphate groups. The existence of the two, nonequivalent phosphate binding sites explains the puzzling formation of, the alternately dephosphorylated myo-inositol triphosphates from phytate, and the hydrolysis of myo-inositol monophosphates.

About this Structure

1H6L is a Single protein structure of sequence from Bacillus amyloliquefaciens with and as ligands. Known structural/functional Sites: , , , , , , , and . Full crystallographic information is available from OCA.

Reference

Enzyme mechanism and catalytic property of beta propeller phytase., Shin S, Ha NC, Oh BC, Oh TK, Oh BH, Structure. 2001 Sep;9(9):851-8. PMID:11566134

Page seeded by OCA on Sun Feb 3 09:47:05 2008