This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1hjk

From Proteopedia

Revision as of 07:49, 3 February 2008 by OCA (Talk | contribs)
Jump to: navigation, search
Image:1hjk.gif

1hjk, resolution 2.3Å

Drag the structure with the mouse to rotate

ALKALINE PHOSPHATASE MUTANT H331Q

Overview

Using a mutant version of E. coli alkaline phosphatase, we succeeded in, trapping and determining the structure of the phospho-enzyme intermediate., The X-ray structure also revealed the catalytic water molecule, bound to, one of the active site zinc ions, positioned ideally for the apical attack, necessary for the hydrolysis of the intermediate.

About this Structure

1HJK is a Single protein structure of sequence from Escherichia coli with , and as ligands. Active as Alkaline phosphatase, with EC number 3.1.3.1 Known structural/functional Sites: and . Full crystallographic information is available from OCA.

Reference

Trapping and visualization of a covalent enzyme-phosphate intermediate., Murphy JE, Stec B, Ma L, Kantrowitz ER, Nat Struct Biol. 1997 Aug;4(8):618-22. PMID:9253408

Page seeded by OCA on Sun Feb 3 09:49:36 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1hjk"
Personal tools