1o6t

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spinqualitylabelsall models 1o6t, resolution 1.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

INTERNALIN (INLA,LISTERIA MONOCYTOGENES)- FUNCTIONAL DOMAIN,UNCOMPLEXED

Overview

Listeria monocytogenes, a food-borne bacterial pathogen, enters mammalian, cells by inducing its own phagocytosis. The listerial protein internalin, (InlA) mediates bacterial adhesion and invasion of epithelial cells in the, human intestine through specific interaction with its host cell receptor, E-cadherin. We present the crystal structures of the functional domain of, InlA alone and in a complex with the extracellular, N-terminal domain of, human E-cadherin (hEC1). The leucine rich repeat (LRR) domain of InlA, surrounds and specifically recognizes hEC1. Individual interactions were, probed by mutagenesis and analytical ultracentrifugation. These include, Pro16 of hEC1, a major determinant for human susceptibility to L., monocytogenes infection that is essential for intermolecular recognition., Our studies reveal the structural basis for host tro-pism of this, bacterium and the molecular deception L. monocytogenes employs to exploit, the E-cadherin system.

About this Structure

1O6T is a Single protein structure of sequence from Listeria monocytogenes with , , , , and as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.

Reference

Structure of internalin, a major invasion protein of Listeria monocytogenes, in complex with its human receptor E-cadherin., Schubert WD, Urbanke C, Ziehm T, Beier V, Machner MP, Domann E, Wehland J, Chakraborty T, Heinz DW, Cell. 2002 Dec 13;111(6):825-36. PMID:12526809

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