1upn

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COMPLEX OF ECHOVIRUS TYPE 12 WITH DOMAINS 3 AND 4 OF ITS RECEPTOR DECAY ACCELERATING FACTOR (CD55) BY CRYO ELECTRON MICROSCOPY AT 16 A

Contents 1 Overview 2 Disease 3 About this Structure 4 Reference

Overview

Echovirus type 12 (EV12), an Enterovirus of the Picornaviridae family, uses the complement regulator decay-accelerating factor (DAF, CD55) as a, cellular receptor. We have calculated a three-dimensional reconstruction, of EV12 bound to a fragment of DAF consisting of short consensus repeat, domains 3 and 4 from cryo-negative stain electron microscopy data (EMD, code 1057). This shows that, as for an earlier reconstruction of the, related echovirus type 7 bound to DAF, attachment is not within the viral, canyon but occurs close to the 2-fold symmetry axes. Despite this general, similarity our reconstruction reveals a receptor interaction that is quite, different from that observed for EV7. Fitting of the crystallographic, co-ordinates for DAF(34) and EV11 into the reconstruction shows a close, agreement between the crystal structure of the receptor fragment and the, density for the virus-bound receptor, allowing unambiguous positioning of, the receptor with respect to the virion (PDB code 1UPN). Our finding that, the mode of virus-receptor interaction in EV12 is distinct from that seen, for EV7 raises interesting questions regarding the evolution and, biological significance of the DAF binding phenotype in these viruses.

Disease

Known diseases associated with this structure: Blood group Cromer OMIM:[125240], Blood group, Knops system OMIM:[120620], CR1 deficiency OMIM:[120620], Malaria, severe, resistance to OMIM:[120620], SLE susceptibility OMIM:[120620]

About this Structure

1UPN is a Protein complex structure of sequences from Homo sapiens and Human echovirus 11. Known structural/functional Site: . Full crystallographic information is available from OCA.

Reference

The structure of echovirus type 12 bound to a two-domain fragment of its cellular attachment protein decay-accelerating factor (CD 55)., Bhella D, Goodfellow IG, Roversi P, Pettigrew D, Chaudhry Y, Evans DJ, Lea SM, J Biol Chem. 2004 Feb 27;279(9):8325-32. Epub 2003 Nov 21. PMID:14634014

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