1uxd
From Proteopedia
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FRUCTOSE REPRESSOR DNA-BINDING DOMAIN, NMR, 34 STRUCTURES
Overview
FruR is an Escherichia coli transcriptional regulator that belongs to the, LacI DNA-binding protein family. By using 1H and 15N NMR spectroscopy, we, have determined the three-dimensional solution structure of the FruR, N-terminal DNA-binding domain consisting of 57 amino acid residues. A, total of 809 NMR-derived distances and 54 dihedral angle constraints have, been used for molecular modelling with the X-PLOR program. The resulting, set of calculated structures presents an average root-mean-square, deviation of 0.37 A at the main-chain level for the first 47 residues., This highly defined N-terminal part of the structure reveals a similar, topology for the three alpha-helices when compared to the 3D structures of, LacI and PurR counterparts. The most striking difference lies in the, connection between helix II and helix III, in which three additional, residues are present in FruR. This connecting segment is well structured, and contains a type III turn. Apart from hydrophobic interactions of, non-polar residues with the core of the domain, this connecting segment is, stabilised by several hydrogen bonds and by the aromatic ring stacking, between Tyr19 of helix II and Tyr28 of the turn. The region containing the, putative "hinge helix" (helix IV), that has been described in PurR-DNA, complex to make specific base contacts in the minor groove of DNA, is, unfolded. Examination of hydrogen bonds highlights the importance of, homologous residues that seem to be conserved for their ability to fulfill, helix N and C-capping roles in the LacI repressor family.
About this Structure
1UXD is a Single protein structure of sequence from Escherichia coli. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Three-dimensional structure of the DNA-binding domain of the fructose repressor from Escherichia coli by 1H and 15N NMR., Penin F, Geourjon C, Montserret R, Bockmann A, Lesage A, Yang YS, Bonod-Bidaud C, Cortay JC, Negre D, Cozzone AJ, Deleage G, J Mol Biol. 1997 Jul 18;270(3):496-510. PMID:9237914
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