ToxT
From Proteopedia
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Introduction
ToxT is a molecule at the end of a transcriptional cascade that autoregulates the transcription of the primary virulence factors of Vibrio cholerae[1] and itself. These two factors, cholera toxin (CT)[2] and the toxin co-regulated pilus (TCP), are instrumental in causing the disease cholera[3]. This is an intestinal infection resulting in massive water loss in the affected individual, causing extreme dehydration.[4]
Structural Features
ToxT belongs to a family of transcriptional regulators known as AraC.[1] The AraC family is defined by a 100 amino acid region of sequence similarity that forms a DNA-binding domain with two helix-turn-helix motifs. [2]
A nine-stranded beta sheet sandwich contains a binding pocket that contains cis-palmitoleate, [1] which appears to have a negative effect on virulence when present in vitro. This unsaturated fatty acid, like other UFAs,[5] tend to inhibit genes under the control of ToxT.
References
- ↑ 1.0 1.1 Lowden MJ, Skorupski K, Pellegrini M, Chiorazzo MG, Taylor RK, Kull FJ. Structure of Vibrio cholerae ToxT reveals a mechanism for fatty acid regulation of virulence genes. Proc Natl Acad Sci U S A. 2010 Feb 16;107(7):2860-5. Epub 2010 Feb 1. PMID:20133655
- ↑ Martin RG, Rosner JL. The AraC transcriptional activators. Curr Opin Microbiol. 2001 Apr;4(2):132-7. PMID:11282467
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