1qr5
From Proteopedia
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SOLUTION STRUCTURE OF HISTIDINE CONTAINING PROTEIN (HPR) FROM STAPHYLOCOCCUS CARNOSUS
Overview
The pressure-induced changes in 15N enriched HPr from Staphylococcus, carnosus were investigated by two-dimensional (2D) heteronuclear NMR, spectroscopy at pressures ranging from atmospheric pressure up to 200 MPa., The NMR experiments allowed the simultaneous observation of the backbone, and side-chain amide protons and nitrogens. Most of the resonances shift, downfield with increasing pressure indicating generalized pressure-induced, conformational changes. The average pressure-induced shifts for amide, protons and nitrogens are 0.285 ppm GPa(-1) at 278 K and 2.20 ppm GPa(-1), respectively. At 298 K the corresponding values are 0.275 and 2.41 ppm, GPa(-1). Proton and nitrogen pressure coefficients show a significant but, rather small correlation (0.31) if determined for all amide ... [(full description)]
About this Structure
1QR5 is a [Single protein] structure of sequence from [Staphylococcus carnosus]. Full crystallographic information is available from [OCA].
Reference
15N and 1H NMR study of histidine containing protein (HPr) from Staphylococcus carnosus at high pressure., Kalbitzer HR, Gorler A, Li H, Dubovskii PV, Hengstenberg W, Kowolik C, Yamada H, Akasaka K, Protein Sci. 2000 Apr;9(4):693-703. PMID:10794411
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