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1ofc

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Revision as of 10:48, 30 October 2007 by OCA (Talk | contribs)
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1ofc, resolution 1.90Å

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NUCLEOSOME RECOGNITION MODULE OF ISWI ATPASE

Overview

Energy-dependent nucleosome remodeling emerges as a key process endowing, chromatin with dynamic properties. However, the principles by which, remodeling ATPases interact with their nucleosome substrate to alter, histone-DNA interactions are only poorly understood. We have identified a, substrate recognition domain in the C-terminal half of the remodeling, ATPase ISWI and determined its structure by X-ray crystallography. The, structure comprises three domains, a four-helix domain with a novel fold, and two alpha-helical domains related to the modules of c-Myb, SANT and, SLIDE, which are linked by a long helix. An integrated structural and, functional analysis of these domains provides insight into how ISWI, interacts with the nucleosomal substrate.

About this Structure

1OFC is a [Single protein] structure of sequence from [Drosophila melanogaster] with GLC, G4D and GOL as [ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

Reference

Crystal structure and functional analysis of a nucleosome recognition module of the remodeling factor ISWI., Grune T, Brzeski J, Eberharter A, Clapier CR, Corona DF, Becker PB, Muller CW, Mol Cell. 2003 Aug;12(2):449-60. PMID:14536084

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