2ret
From Proteopedia
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The crystal structure of a binary complex of two pseudopilins: EpsI and EpsJ from the Type 2 Secretion System of Vibrio vulnificus
Overview
Type II secretion systems (T2SS) translocate virulence factors from the, periplasmic space of many pathogenic bacteria into the extracellular, environment. The T2SS of Vibrio cholerae and related species is called the, extracellular protein secretion (Eps) system that consists of a core of, multiple copies of 11 different proteins. The pseudopilins, EpsG, EpsH, EpsI, EpsJ and EpsK, are five T2SS proteins that are thought to assemble, into a pseudopilus, which is assumed to interact with the outer membrane, pore, and may actively participate in the export of proteins. We report, here biochemical evidence that the minor pseudopilins EpsI and EpsJ from, Vibrio species interact directly with one another. Moreover, the 2.3 A, resolution crystal structure of a complex of EspI and EpsJ from Vibrio, vulnificus represents the first atomic resolution structure of a complex, of two different pseudopilin components from the T2SS. Both EpsI and EpsJ, appear to be structural extremes within the family of type 4a pilin, structures solved to date, with EpsI having the smallest, and EpsJ the, largest, "variable pilin segment" seen thus far. A high degree of sequence, conservation in the EpsI:EpsJ interface indicates that this heterodimer, occurs in the T2SS of a large number of bacteria. The arrangement of EpsI, and EpsJ in the heterodimer would correspond to a right-handed helical, character of proteins assembled into a pseudopilus.
About this Structure
2RET is a Protein complex structure of sequences from Vibrio vulnificus with and as ligands. Known structural/functional Sites: , , and . Full crystallographic information is available from OCA.
Reference
The crystal structure of a binary complex of two pseudopilins: EpsI and EpsJ from the type 2 secretion system of Vibrio vulnificus., Yanez ME, Korotkov KV, Abendroth J, Hol WG, J Mol Biol. 2008 Jan 11;375(2):471-86. Epub 2007 Oct 22. PMID:18022192
Page seeded by OCA on Wed Feb 13 08:14:36 2008
