1csl
From Proteopedia
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CRYSTAL STRUCTURE OF THE RRE HIGH AFFINITY SITE
Overview
The crystal structure of a 28 nt RNA fragment containing the human, immunodeficiency virus type 1 (HIV-1) Rev response element high affinity, binding site for Rev protein has been solved at 1.6 A resolution. The, overall structure of the RRE helix is greatly distorted from A-form, geometry by the presence of two purine-purine base-pairs and two single, nucleotide bulges. G48 and G71 form a Hoogsteen-type asymmetric base-pair, with G71 adopting a syn conformation. The non-canonical regions in the, unliganded Rev response element molecule narrow the major groove width, with respect to standard A-RNA. The Rev response element structure, observed here represents a closed form of the Rev binding site and differs, from conformations of the RNA observed previously by solution NMR studies.
About this Structure
1CSL is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
The structure of the HIV-1 RRE high affinity rev binding site at 1.6 A resolution., Ippolito JA, Steitz TA, J Mol Biol. 2000 Jan 28;295(4):711-7. PMID:10656783
Page seeded by OCA on Fri Feb 15 15:37:01 2008
