Horseradish peroxidase
From Proteopedia
Horseradish peroxidase (HRP) catalyzes the oxidation of luminol to 3-aminophthalate using hydrogen peroxide. The reaction is accompanied by emission of light. In the presence of chemicals, particularly – p-iodophenol, the light emission is enhanced 1000-fold. HRP isozyme C1a is widely used for detection of a labeled molecule to which it conjugates. Upon addition of the proper substrate, the HRP complex emits colored light. HRP contains a heme group.
3D structures of horseradish peroxidase
1atj, 1h5d, 1h5e, 1h5f, 1h5g, 1g5i, 1g5j, 1g5k, 1g5m, 1g57, 1h55, 1h58, 1h5c, 1h5h, 1h5l, 1hch – HRP C1a
1kzm, 1gwo, 1gwt, 1gwu - HRP C1a (mutant)
Horseradish peroxidase binary complex
2atj – HRP C1a + benzhydroxamic acid
3atj, 4atj, 1gx2 - HRP C1a (mutant) + benzhydroxamic acid
6atj - HRP C1a + ferulic acid
1gw2 - HRP C1a (mutant) + ferulic acid
7atj - HRP C1a + CN + ferulic acid
1h5a – HRP C1a + acetate
1w4w - HRP C1a + formate
1w4w - HRP C1a + CO
