1op9

From Proteopedia

Complex of human lysozyme with camelid VHH HL6 antibody fragment

Overview

Amyloid diseases are characterized by an aberrant assembly of a specific, protein or protein fragment into fibrils and plaques that are deposited in, various organs and tissues, often with serious pathological consequences., Non-neuropathic systemic amyloidosis is associated with single point, mutations in the gene coding for human lysozyme. Here we report that a, single-domain fragment of a camelid antibody raised against wild-type, human lysozyme inhibits the in vitro aggregation of its amyloidogenic, variant, D67H. Our structural studies reveal that the epitope includes, neither the site of mutation nor most residues in the region of the, protein structure that is destabilized by the mutation. Instead, the, binding of the antibody fragment achieves its effect by restoring the, structural cooperativity characteristic of the wild-type protein. This, appears to occur at least in part through the transmission of long-range, conformational effects to the interface between the two structural domains, of the protein. Thus, reducing the ability of an amyloidogenic protein to, form partly unfolded species can be an effective method of preventing its, aggregation, suggesting approaches to the rational design of therapeutic, agents directed against protein deposition diseases.

Disease

Known diseases associated with this structure: Amyloidosis, renal OMIM:[153450], Microphthalmia, syndromic 1 OMIM:[309800]

About this Structure

1OP9 is a Single protein structure of sequence from Camelus dromedarius and Homo sapiens. Active as Lysozyme, with EC number 3.2.1.17 Full crystallographic information is available from OCA.

Reference

A camelid antibody fragment inhibits the formation of amyloid fibrils by human lysozyme., Dumoulin M, Last AM, Desmyter A, Decanniere K, Canet D, Larsson G, Spencer A, Archer DB, Sasse J, Muyldermans S, Wyns L, Redfield C, Matagne A, Robinson CV, Dobson CM, Nature. 2003 Aug 14;424(6950):783-8. PMID:12917687

Page seeded by OCA on Fri Feb 15 16:34:53 2008