Vibrio cholerae colonization factor TcpF

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Contents 1 Introduction 2 Structure 3 Function 4 Multiply Sequence Alignment 5 Application 6 Reference

Introduction

Molecule: Toxin coregulated pilus biosynthesis protein F

Type:protein Length: 318 Organism:Vibrio Cholerae

TcpF is a toxin-coregulated pilus that facilitates colonization of vibrio cholerae in the intestine. Vibrio cholerae relies on two main virulence factors: toxin-coregulated pilus (TCP) and cholera toxin (CT) to cause the gastrointestinal disease cholera. TCP, encoded by the tcp operon, is a type IV pilus that mediates bacterial autoagglutination and colonization of the intestine.

Structure

The structure of TcpF is consisted with an and a connected by an extended linker segment (residues 186–189). In detail, the NTD is composed of a short twisted β-sheet encapsulated by seven short α-helices with a second twisted β-sheet forming the floor of this domain. The NTD is connected with The CTD, which consists of two twisted antiparallel β-sheets. TCP is a filamentous structure belongs. The architecture of TcpF, with discrete NTD and CTD joined by a linker, is flexible that allow to accommodate a larger substrate. By looking at the homology of TcpF, the NTD and the CTD together form a unique interface that interacts with partner proteins to function in V. cholerae colonization.

Function

TcpF, identified in classical isolates of V. cholerae O1 is an essential factor for colonization in the infant mouse cholera model. Bacteria lacking tcpF are deficient in colonization, and anti-TcpF antibodies are protective in the infant mouse cholera model. TcpF is expressed in vivo during human infection and generates a substantial immune response in patients infected with V. cholera. The mechanism for the action of TcpF remains to be elucidated.

Multiply Sequence Alignment

Application

Reference

[1]. Genetic Mapping of Secretion and Functional Determinants of the Vibrio cholerae TcpF Colonization Factor Shelly J. Krebs, Thomas J. Kirn and Ronald K. Taylor J. Bacteriol. 2009, 191(11):3665. DOI: 10.1128/JB.01724-08. Published Ahead of Print 20 March 2009. [2]. TcpF Is a Soluble Colonization Factor and Protective Antigen Secreted by El Tor and Classical O1 and O139 Vibrio cholerae Serogroups Thomas J. Kirn and Ronald K. Taylor DOI: 10.1128/​IAI.73.8.4461-4470.2005 Infect. Immun. August 2005 vol. 73 no. 8 4461-4470. [3]. Crystal structure of the Vibrio cholerae colonization factor TcpF and identification of a functional immunogenic site. Megli CJ, Yuen AS, Kolappan S, Richardson MR, Dharmasena MN, Krebs SJ, Taylor RK, Craig L. J Mol Biol. 2011 Jun 3;409(2):146-58. Epub 2011 Apr 1.