1w4m

From Proteopedia

proteopedia linkproteopedia link

spinqualitylabelsall models 1w4m Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

STRUCTURE OF THE HUMAN PLECKSTRIN DEP DOMAIN BY MULTIDIMENSIONAL NMR

Contents 1 Overview 2 Disease 3 About this Structure 4 Reference

Overview

Pleckstrin1 is a major substrate for protein kinase C in platelets and, leukocytes, and comprises a central DEP (disheveled, Egl-10, pleckstrin), domain, which is flanked by two PH (pleckstrin homology) domains. DEP, domains display a unique alpha/beta fold and have been implicated in, membrane binding utilizing different mechanisms. Using multiple sequence, alignments and phylogenetic tree reconstructions, we find that 6, subfamilies of the DEP domain exist, of which pleckstrin represents a, novel and distinct subfamily. To clarify structural determinants of the, DEP fold and to gain further insight into the role of the DEP domain, we, determined the three-dimensional structure of the pleckstrin DEP domain, using heteronuclear NMR spectroscopy. Pleckstrin DEP shares main, structural features with the DEP domains of disheveled and Epac, which, belong to different DEP subfamilies. However, the pleckstrin DEP fold is, distinct from these structures and contains an additional, short helix, alpha4 inserted in the beta4-beta5 loop that exhibits increased backbone, mobility as judged by NMR relaxation measurements. Based on sequence, conservation, the helix alpha4 may also be present in the DEP domains of, regulator of G-protein signaling (RGS) proteins, which are members of the, same DEP subfamily. In pleckstrin, the DEP domain is surrounded by two PH, domains. Structural analysis and charge complementarity suggest that the, DEP domain may interact with the N-terminal PH domain in pleckstrin., Phosphorylation of the PH-DEP linker, which is required for pleckstrin, function, could regulate such an intramolecular interaction. This suggests, a role of the pleckstrin DEP domain in intramolecular domain interactions, which is distinct from the functions of other DEP domain subfamilies found, so far.

Disease

Known disease associated with this structure: Age-related maculopathy, susceptibility to OMIM:[607772]

About this Structure

1W4M is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structure and dynamics of the human pleckstrin DEP domain: distinct molecular features of a novel DEP domain subfamily., Civera C, Simon B, Stier G, Sattler M, Macias MJ, Proteins. 2005 Feb 1;58(2):354-66. PMID:15573383

Page seeded by OCA on Fri Feb 15 17:05:25 2008