Galactosidase
From Proteopedia
α-Galactosidase (Agal) hydrolyzes the terminal α-galactosyl moiety from glycoproteins and glycolipids. β-Galactosidase hydrolyzes β-galactosides into monosaccharides. Isopropyl-β-d-thiogalactopyranoside (IPTG) induces Bgal activity. Phenylethyl-β-d-thiogalactopyranoside (PETG) is an inhibitor. Galactose, lactose, o-nitrophenyl-β-d-galactoside (ONPG) are substrates. Galactopyranosyl is reaction intermediate. The images at the left and at the right correspond to one representative Galactosidase, i.e. the crystal structure of Galactosidase from Escherichia coli (3e1f). For details on β-galactosidase see Molecular Playground/Beta-galactosidase.
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3D Structures of Galactosidase
Updated June 2012
α-Galactosidase
3lrk – yAgal 1 (mutant) – yeast
3lrl, 3lrm – yAgal 1 (mutant) + polysaccharide
3lx9, 3lxa, 3lxb, 3lxc, 3tv8 - hAgal A (mutant) – human
3hg2, 3gxn, 1r46, 1r47, 3s5y, 3s5z – hAgal A
1zy9 – TmAgal – Thermotoga maritima
3h53 - hAgal B (mutant)
3a24 – BtAgal – Bacterioides thetaiotamicron
3a5v – Agal I – Mortierella vinacea
3mi6 – Agal – Lactobacillus brevis
1szn – HjAgal – Hypocrea jecorina
1uas – Agal – rice
2xn0, 2xn1 – LaAgal – Lactobacillus acidophilus
α-galactosidase binary complex
3h54 - hAgal B (mutant) + polysaccharide
3h55 - hAgal B (mutant) + galactose
3igu - hAgal B (mutant) + intermediate
3hg3, 3hg5 - hAgal A (mutant) + galactose
3gxp - hAgal A + galactose
3hg4 – hAgal A + intermediate
3gxt – hAgal A + imino sugar
1t0o – HjAgal + galactose
2xn2 - LaAgal + galactose
β-Galactosidase
3og2 – TrBgal – Trichoderma reesei
3muy, 3i3e, 3iap, 3iaq, 3dyp, 1px3, 3sep, 3t0a, 3t2o, 3vd3, 3vd5, 3vda – EcBgal (mutant) – Escherichia coli
1hn1, 1dp0, 1f49, 1f4a, 1f4h, 1gho, 1bgl, 1bgm - EcBgal
3dec, 3d3a, 3bga – BtBgal
1yq2 - Bgal (mutant) – Arthrobacter
1uwi - SsBgal (mutant) - Sulfolobus solfataricus
1uwq – SsBgal
1kwg – TtBgal – Thermus thermophilus
1tg7 – PeBgal – Penicillium
3tts – BcBgal – Bacillus circulans
β-Galactosidase binary complex
1xc6 - PeBgal + galactose
1kwk – TtBgal + galactose
3ogr – TrBgal + galactose
3ogs - TrBgal + IPTG
3ogv – TrBgal + inhibitor
2ceq, 1uwr, 1uws, 1uwt, 1uwu - SsBgal + inhibitor
3muz, 3i3d, 3dyo, 1px4, 1jyx, 3t08, 3t0b, 3t2p, 3vd4, 3vd9, 3vdc- EcBgal (mutant) + IPTG
3mv0, 3i3b, 3czj, 1jz0, 1jz1, 1jz2, 1jz3, 1jz4, 1jz5, 1jz6 - EcBgal (mutant) + intermediate
3mv1 - EcBgal (mutant) + guanidinium
3e1f, 1jz7 - EcBgal (mutant) + galactose
3t09, 3t0d, 3t2q, 3vd7, 3vdb - EcBgal (mutant) + galactose derivative
1jyn, 1jyy, 1jyz, 1jz8 - EcBgal (mutant) + lactose derivative
1jyn, 1jyw, 1jyv - EcBgal (mutant) + substrate
1jz6 - EcBgal (mutant) + inhibitor
3ob8, 3oba – Bgal – Kluyveromyces lactis
3tty - BcBgal + galactose
Endo-1,4-β-galactosidase
2xom, 2xon – TmEbgal carbohydrate-binding domain + β-galactotriose
1ups – Bgal - Clostridium perfringens
2ygl, 2ygm – Bgal blood group A & B cleaving – Streptococcus pneumoniae
3,6-anhydro-α-L-galactosidase
3p2n – AAgal – Zobellia galactanivorans
6-phospho-β-D-galactosidase
2pbg, 3pbg, 4pbg, 1pbg – Pgal (mutant) – Lactococcus lacti
2v4v – gal GH59 – Clostridium cellulolyticum
