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2bly
From Proteopedia
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HEWL AFTER A HIGH DOSE X-RAY "BURN"
Overview
Specific radiation damage can be used to solve macromolecular structures, using the radiation-damage-induced phasing (RIP) method. The method has, been investigated for six disulfide-containing test structures (elastase, insulin, lysozyme, ribonuclease A, trypsin and thaumatin) using data sets, that were collected on a third-generation synchrotron undulator beamline, with a highly attenuated beam. Each crystal was exposed to the, unattenuated X-ray beam between the collection of a 'before' and an, 'after' data set. The X-ray 'burn'-induced intensity differences ranged, from 5 to 15%, depending on the protein investigated. X-ray-susceptible, substructures were determined using the integrated direct and Patterson, methods in SHELXD. The best substructures were found by downscaling the, ... [(full description)]
About this Structure
2BLY is a [Single protein] structure of sequence from [Gallus gallus] with PG4 as [ligand]. Active as [Lysozyme], with EC number [3.2.1.17]. Structure known Active Site: AC2. Full crystallographic information is available from [OCA].
Reference
Improving radiation-damage substructures for RIP., Nanao MH, Sheldrick GM, Ravelli RB, Acta Crystallogr D Biol Crystallogr. 2005 Sep;61(Pt 9):1227-37. Epub 2005, Aug 16. PMID:16131756
Page seeded by OCA on Tue Oct 30 13:52:11 2007
