1spu

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spinqualitylabelsall models 1spu, resolution 2.0Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

STRUCTURE OF OXIDOREDUCTASE

Overview

The crystal structure of the complex between the copper amine oxidase from, Escherichia coli (ECAO) and a covalently bound inhibitor, 2-hydrazinopyridine, has been determined to a resolution of 2.0 A. The, inhibitor covalently binds at the 5 position of the quinone ring of the, cofactor, 2,4,5-trihydroxyphenylalaninequinone (TPQ). The inhibitor, complex is analogous to the substrate Schiff base formed during the, reaction with natural monoamine substrate. A proton is abstracted from a, methylene group adjacent to the amine group by a catalytic base during the, reaction. The inhibitor, however, has a nitrogen at this position, preventing proton abstraction and trapping the enzyme in a covalent, complex. The electron density shows this nitrogen is hydrogen bonded to, the side chain of ... [(full description)]

About this Structure

1SPU is a [Single protein] structure of sequence from [Escherichia coli] with CU and CA as [ligands]. Active as [[1]], with EC number [1.4.3.6]. Full crystallographic information is available from [OCA].

Reference

Catalytic mechanism of the quinoenzyme amine oxidase from Escherichia coli: exploring the reductive half-reaction., Wilmot CM, Murray JM, Alton G, Parsons MR, Convery MA, Blakeley V, Corner AS, Palcic MM, Knowles PF, McPherson MJ, Phillips SE, Biochemistry. 1997 Feb 18;36(7):1608-20. PMID:9048544

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