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1gcy
From Proteopedia
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HIGH RESOLUTION CRYSTAL STRUCTURE OF MALTOTETRAOSE-FORMING EXO-AMYLASE
Overview
The intact maltotetraose-forming exo-amylase from Pseudomonas stutzeri (G4-1), which has a raw starch binding domain, has been crystallized. The structure was identified (PDB entry 1GCY) by the molecular replacement method using the structure of its catalytic domain (G4-2). The result showed that the raw starch binding domain is in a disordered state, the corresponding electron densities being almost invisible. Superposition of these two enzyme forms showed evidence for the possible location of the raw starch binding domain (SBD). This crystal is a novel case, in that it forms a regular lattice incorporating flexibly bound SBD in the channel of crystal packing of the catalytic domains.
About this Structure
1GCY is a Single protein structure of sequence from Pseudomonas stutzeri with as ligand. Active as Glucan 1,4-alpha-maltotetraohydrolase, with EC number 3.2.1.60 Full crystallographic information is available from OCA.
Reference
Crystallization and structural analysis of intact maltotetraose-forming exo-amylase from Pseudomonas stutzeri., Mezaki Y, Katsuya Y, Kubota M, Matsuura Y, Biosci Biotechnol Biochem. 2001 Jan;65(1):222-5. PMID:11272837
Page seeded by OCA on Thu Feb 21 12:48:45 2008
