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1ik3

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Revision as of 11:12, 21 February 2008 by OCA (Talk | contribs)
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1ik3, resolution 2.0Å

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LIPOXYGENASE-3 (SOYBEAN) COMPLEX WITH 13(S)-HYDROPEROXY-9(Z),11(E)-OCTADECADIENOIC ACID

Overview

Polyunsaturated fatty acid metabolism is governed primarily by two enzymes, prostaglandin H synthase and lipoxygenase. The crystal structure of the metastable product-oxidized purple form of soybean lipoxygenase-3 was determined at 2.0 A resolution. The data reveal that the chromophore corresponds to an iron-peroxide complex, a potential intermediate in the catalyzed reaction. A significant alteration of the iron site accompanies the formation of the complex. The structure, the first for a fatty acid-lipoxygenase complex, also reveals an unexpected mode of binding, and identifies amino acid residues that may play significant roles in catalysis, regio- and stereoselectivity.

About this Structure

1IK3 is a Single protein structure of sequence from Glycine max with , , , and as ligands. Active as Lipoxygenase, with EC number 1.13.11.12 Known structural/functional Site: . Full crystallographic information is available from OCA.

Reference

Three-dimensional structure of a purple lipoxygenase., Skrzypczak-Jankun E, Bross RA, Carroll RT, Dunham WR, Funk MO Jr, J Am Chem Soc. 2001 Nov 7;123(44):10814-20. PMID:11686682

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