1jf9
From Proteopedia
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Crystal Structure of selenocysteine lyase
Overview
The Escherichia coli NifS CsdB protein is a member of the homodimeric pyridoxal 5'-phosphate (PLP)-dependent family of enzymes. These enzymes are capable of decomposing cysteine or selenocysteine into L-alanine and sulfur or selenium, respectively. E. coli NifS CsdB has a high specificity for L-selenocysteine in comparison to l-cysteine, suggesting a role for this enzyme is selenium metabolism. The 2.0 A crystal structure of E. coli NifS CsdB reveals a high-resolution view of the active site of this enzyme in apo-, persulfide, perselenide, and selenocysteine-bound intermediates, suggesting a mechanism for the stabilization of the enzyme persulfide and perselenide intermediates during catalysis, a necessary intermediate in the formation of sulfur and selenium containing metabolites.
About this Structure
1JF9 is a Single protein structure of sequence from Escherichia coli with as ligand. Active as Selenocysteine lyase, with EC number 4.4.1.16 Full crystallographic information is available from OCA.
Reference
Analysis of the E. coli NifS CsdB protein at 2.0 A reveals the structural basis for perselenide and persulfide intermediate formation., Lima CD, J Mol Biol. 2002 Feb 1;315(5):1199-208. PMID:11827487
Page seeded by OCA on Thu Feb 21 13:22:00 2008
Categories: Escherichia coli | Selenocysteine lyase | Single protein | Burley, S K. | Lima, C D. | NYSGXRC, New York Structural GenomiX Research Consortium. | PLP | Cysteine | New york structural genomix research consortium | Nifs | Nysgxrc | Protein structure initiative | Psi | Pursulfide | Selenocysteine | Structural genomics
