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1jxg
From Proteopedia
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The 1.6 A Resolution Crystal Structure of a Mutant Poplar Plastocyanin Bearing a 21-25 Engeneered Disulfide Bridge
Overview
Plastocyanin is an electron-transfer protein which has been largely used for biophysical studies as well as for protein-engineering experiments. A surface disulfide bridge has been engineered in poplar plastocyanin to allow protein chemisorption on gold substrates. The mutated plastocyanin crystal structure has been studied at 1.6 A resolution (R factor = 0.145, R(free) = 0.205) to characterize the effects of the engineered disulfide on the overall protein structure and on the Cu-coordination sphere in view of biophysical applications. The new orthorhombic crystal form isolated for the mutated plastocyanin displays two protein molecules per asymmetric unit.
About this Structure
1JXG is a Single protein structure of sequence from Populus nigra with and as ligands. Full crystallographic information is available from OCA.
Reference
The 1.6 A resolution crystal structure of a mutant plastocyanin bearing a 21-25 engineered disulfide bridge., Milani M, Andolfi L, Cannistraro S, Verbeet MP, Bolognesi M, Acta Crystallogr D Biol Crystallogr. 2001 Nov;57(Pt 11):1735-8. Epub 2001, Oct 25. PMID:11679761
Page seeded by OCA on Thu Feb 21 13:27:48 2008
Categories: Populus nigra | Single protein | Andolfi, L. | Bolognesi, M. | Cannistraro, S. | Milani, M. | Verbeet, M P. | CU1 | SO4 | Beta barrel
