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2v63
From Proteopedia
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CRYSTAL STRUCTURE OF RUBISCO FROM CHLAMYDOMONAS REINHARDTII WITH A LARGE-SUBUNIT V331A MUTATION
Overview
The loop between alpha-helix 6 and beta-strand 6 in the alpha/beta-barrel, of ribulose-1,5-bisphosphate carboxylase/oxygenase plays a key role in, discriminating between CO2 and O2. Genetic screening in Chlamydomonas, reinhardtii previously identified a loop-6 V331A substitution that, decreases carboxylation and CO2/O2 specificity. Revertant selection, identified T342I and G344S substitutions that restore photosynthetic, growth by increasing carboxylation and specificity of the V331A enzyme. In, numerous X-ray crystal structures, loop 6 is closed or open depending on, the activation state of the enzyme and the presence or absence of ligands., The carboxy terminus folds over loop 6 in the closed state. To study the, molecular basis for catalysis, directed mutagenesis and chloroplast, ... [(full description)]
About this Structure
2V63 is a [Protein complex] structure of sequences from [Chlamydomonas reinhardtii] with MG, CAP and EDO as [ligands]. Active as [Ribulose-bisphosphate carboxylase], with EC number [4.1.1.39]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Structural Analysis of Altered Large-Subunit Loop-6/Carboxy-Terminus Interactions That Influence Catalytic Efficiency and CO(2)/O(2) Specificity of Ribulose-1,5-bisphosphate Carboxylase/Oxygenase(,)., Karkehabadi S, Satagopan S, Taylor TC, Spreitzer RJ, Andersson I, Biochemistry. 2007 Oct 2;46(39):11080-9. Epub 2007 Sep 8. PMID:17824672
Page seeded by OCA on Tue Oct 30 14:32:14 2007
Categories: Chlamydomonas reinhardtii | Protein complex | Ribulose-bisphosphate carboxylase | Andersson, I. | Karkehabadi, S. | Satagopan, S. | Spreitzer, R.J. | Taylor, T.C. | CAP | EDO | MG | Acetylation | Calvin cycle | Carbon dioxide fixation | Chloroplast | Co2/o2 specificity | Hydroxylation | Large subunit loop 6 mutation | Lyase | Magnesium | Metal-binding | Methylation | Monooxygenase | Oxidoreductase | Photorespiration | Photosynthesis | Plastid | Rubisco | Transit peptide
