1m1f
From Proteopedia
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Kid toxin protein from E.coli plasmid R1
Overview
We have determined the structure of Kid toxin protein from E. coli plasmid R1 involved in stable plasmid inheritance by postsegregational killing of plasmid-less daughter cells. Kid forms a two-component system with its antagonist, Kis antitoxin. Our 1.4 A crystal structure of Kid reveals a 2-fold symmetric dimer that closely resembles the DNA gyrase-inhibitory toxin protein CcdB from E. coli F plasmid despite the lack of any notable sequence similarity. Analysis of nontoxic mutants of Kid suggests a target interaction interface associated with toxicity that is in marked contrast to that proposed for CcdB. A possible region for interaction of Kid with the antitoxin is proposed that overlaps with the target binding site and may explain the mode of antitoxin action.
About this Structure
1M1F is a Single protein structure of sequence from Escherichia coli with as ligand. Full crystallographic information is available from OCA.
Reference
Structural and functional analysis of the kid toxin protein from E. coli plasmid R1., Hargreaves D, Santos-Sierra S, Giraldo R, Sabariegos-Jareno R, de la Cueva-Mendez G, Boelens R, Diaz-Orejas R, Rafferty JB, Structure. 2002 Oct;10(10):1425-33. PMID:12377128
Page seeded by OCA on Thu Feb 21 13:50:33 2008
Categories: Escherichia coli | Single protein | Boelens, R. | Cueva-Mendez, G de la. | Diaz-Orejas, R. | Giraldo, R. | Hargreaves, D. | Rafferty, J B. | Sabariegos-Jareno, R. | Santos-Sierra, S. | PO4 | Ccdb | Dna replication | Mutational analysis | Plasmid maintenance | Post segregational killing | Toxin-antitoxin
