1ayx
From Proteopedia
|
CRYSTAL STRUCTURE OF GLUCOAMYLASE FROM SACCHAROMYCOPSIS FIBULIGERA AT 1.7 ANGSTROMS
Overview
The yeast Saccharomycopsis fibuligera produces a glucoamylase which, belongs to sequence family 15 of glycosyl hydrolases. The structure of the, non-glycosyl-ated recombinant enzyme has been determined by molecular, replacement and refined against 1.7 A resolution synchrotron data to an R, factor of 14.6%. This is the first report of the three-dimensional, structure of a yeast family 15 glucoamylase. The refinement from the, initial molecular-replacement model was not straightforward. It involved, the use of an unrestrained automated refinement procedure (uARP) in, combination with the maximum-likelihood refinement program REFMAC. The, enzyme consists of 492 amino-acid residues and has 14 alpha-helices, 12 of, which form an (alpha/alpha)6 barrel. It contains a single catalytic domain, but ... [(full description)]
About this Structure
1AYX is a [Single protein] structure of sequence from [Saccharomycopsis fibuligera] with TRS as [ligand]. Active as [Glucan 1,4-alpha-glucosidase], with EC number [3.2.1.3]. Structure known Active Site: NUL. Full crystallographic information is available from [OCA].
Reference
Structure of glucoamylase from Saccharomycopsis fibuligera at 1.7 A resolution., Sevcik J, Solovicova A, Hostinova E, Gasperik J, Wilson KS, Dauter Z, Acta Crystallogr D Biol Crystallogr. 1998 Sep 1;54(Pt 5):854-66. PMID:9757101
Page seeded by OCA on Tue Oct 30 14:52:59 2007
