This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1s2r
From Proteopedia
|
A High Resolution Crystal Structure of [d(CGCAAATTTGCG)]2
Overview
The crystal structure of [d(CGCAAATTTGCG)]2 has been determined to 1.5 A resolution, representing the first high-resolution structure of this DNA fragment. The ion interactions are novel. A spermine molecule replaces a Mg2+ observed in analogous structures. Unlike lower-resolution structures, the minor groove is narrow and the major groove lacks extra Watson-Crick hydrogen bonds. In addition, a monolayer of solvent sites, including a "spine of hydration", is visible in the minor groove. The crystal of [d(CGCAAATTTGCG)]2 was grown from a solution containing spermine, magnesium, and lithium. The conformation recapitulates that of "monovalent-minus" DNA.
About this Structure
1S2R is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
High-resolution structure of an extended A-tract: [d(CGCAAATTTGCG)]2., Woods KK, Maehigashi T, Howerton SB, Sines CC, Tannenbaum S, Williams LD, J Am Chem Soc. 2004 Dec 1;126(47):15330-1. PMID:15563130
Page seeded by OCA on Thu Feb 21 14:57:16 2008
