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Thermotoga maritima β-fructosidase (invertase)
Introduction
The β-fructosidase (β-D-fructofuranosidase EC 3. 2. 1. 26.) of the thermophilic baterium Thermotoga maritima is an enzyme which hydrolyzes sucrose to release an equimolar mixture of fructose and glucose, called inberted sugar. That is why the other name of the β-fructosidase is invertase. 
This enzyme achieves the hydrolysis with a retention molecular mechanism. Actually, the invertase hydrolyses the D-sucrose to form the D-fructose and the D-glucose. So, the substrate anomeric configuration is conserved in products. The β-fructosidase belongs to the GH32 (Glycoside hydrolysases 32) according to the sequence-based classification of glycoside hydrolysases. The β-fructosidase hydrolyses other substrates like raffinose or stachyose but here we will focus on sucrose hysrolyse.
Glycoside Hydrolases and GH32 family
Glycoside Hydrolysases
Glycoside hydrolases (GH)[1]
