1tip
From Proteopedia
|
THE BISPHOSPHATASE DOMAIN OF THE BIFUNCTIONAL RAT LIVER 6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE
Overview
The crystal structure of the fructose-2,6-bisphosphatase domain trapped during the reaction reveal a phosphorylated His 258, and a water molecule immobilized by the product, fructose-6-phosphate. The geometry suggests that the dephosphorylation step requires prior removal of the product for an 'associative in-line' phosphoryl transfer to the catalytic water.
About this Structure
1TIP is a Single protein structure of sequence from Rattus norvegicus with as ligand. Active as Fructose-2,6-bisphosphate 2-phosphatase, with EC number 3.1.3.46 Full crystallographic information is available from OCA.
Reference
Crystal structure of a trapped phosphoenzyme during a catalytic reaction., Lee YH, Olson TW, Ogata CM, Levitt DG, Banaszak LJ, Lange AJ, Nat Struct Biol. 1997 Aug;4(8):615-8. PMID:9253407
Page seeded by OCA on Thu Feb 21 15:13:57 2008
Categories: Fructose-2,6-bisphosphate 2-phosphatase | Rattus norvegicus | Single protein | Banaszak, L J. | Lange, A J. | Lee, Y H. | Levitt, D G. | Ogata, C M. | Olson, T W. | F6P | Alternative splicing | Atp-binding | Hydrolase | Kinase | Multifunctional enzyme | Multigene family | Phosphorylation | Transferase
