1gmm
From Proteopedia
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CARBOHYDRATE BINDING MODULE CBM6 FROM XYLANASE U CLOSTRIDIUM THERMOCELLUM
Overview
Polysaccharide-degrading enzymes are generally modular proteins that, contain non-catalytic carbohydrate-binding modules (CBMs), which, potentiate the activity of the catalytic module. CBMs have been grouped, into sequence-based families, and three-dimensional structural data are, available for half of these families. Clostridium thermocellum xylanase, 11A is a modular enzyme that contains a CBM from family 6 (CBM6), for, which no structural data are available. We have determined the crystal, structure of this module to a resolution of 2.1 A. The protein is a, beta-sandwich that contains two potential ligand-binding clefts designated, cleft A and B. The CBM interacts primarily with xylan, and NMR, spectroscopy coupled with site-directed mutagenesis identified cleft A, containing Trp-92, ... [(full description)]
About this Structure
1GMM is a [Single protein] structure of sequence from [Clostridium thermocellum] with SO4, NA and CA as [ligands]. Active as [Endo-1,4-beta-xylanase], with EC number [3.2.1.8]. Structure known Active Site: CA1. Full crystallographic information is available from [OCA].
Reference
The location of the ligand-binding site of carbohydrate-binding modules that have evolved from a common sequence is not conserved., Czjzek M, Bolam DN, Mosbah A, Allouch J, Fontes CM, Ferreira LM, Bornet O, Zamboni V, Darbon H, Smith NL, Black GW, Henrissat B, Gilbert HJ, J Biol Chem. 2001 Dec 21;276(51):48580-7. Epub 2001 Oct 22. PMID:11673472
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