1h1x

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spinqualitylabelsall models 1h1x, resolution 1.4Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

SPERM WHALE MYOGLOBIN MUTANT T67R S92D

Overview

Atomic co-ordinates and structure factors for the T67R/S92D metMbCN mutant, have been deposited with the Protein Data Bank, under accession codes 1h1x, and r1h1xsf, respectively. Protein engineering and cofactor replacement, have been employed as tools to introduce/modulate peroxidase activity in, sperm whale Mb (myoglobin). Based on the rationale that haem peroxidase, active sites are characterized by specific charged residues, the Mb haem, crevice has been modified to host a haem-distalpropionate Arg residue and, a proximal Asp, yielding the T67R/S92D Mb mutant. To code extra, conformational mobility around the haem, and to increase the peroxidase, catalytic efficiency, the T67R/S92D Mb mutant has been subsequently, reconstituted with protohaem-L-histidine methyl ester, yielding a ... [(full description)]

About this Structure

1H1X is a [Single protein] structure of sequence from [Physeter catodon] with CYN, SO4 and HEM as [ligands]. Structure known Active Site: HEM. Full crystallographic information is available from [OCA].

Reference

Engineering peroxidase activity in myoglobin: the haem cavity structure and peroxide activation in the T67R/S92D mutant and its derivative reconstituted with protohaemin-l-histidine., Roncone R, Monzani E, Murtas M, Battaini G, Pennati A, Sanangelantoni AM, Zuccotti S, Bolognesi M, Casella L, Biochem J. 2004 Feb 1;377(Pt 3):717-24. PMID:14563209

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