1xsf
From Proteopedia
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Solution structure of a resuscitation promoting factor domain from Mycobacterium tuberculosis
Overview
Resuscitation-promoting factor (RPF) proteins reactivate stationary-phase cultures of (G+C)-rich Gram-positive bacteria including the causative agent of tuberculosis, Mycobacterium tuberculosis. We report the solution structure of the RPF domain from M. tuberculosis Rv1009 (RpfB) solved by heteronuclear multidimensional NMR. Structural homology with various glycoside hydrolases suggested that RpfB cleaved oligosaccharides. Biochemical studies indicate that a conserved active site glutamate is important for resuscitation activity. These data, as well as the presence of a clear binding pocket for a large molecule, indicate that oligosaccharide cleavage is probably the signal for revival from dormancy.
About this Structure
1XSF is a Single protein structure of sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA.
Reference
The structure of a resuscitation-promoting factor domain from Mycobacterium tuberculosis shows homology to lysozymes., Cohen-Gonsaud M, Barthe P, Bagneris C, Henderson B, Ward J, Roumestand C, Keep NH, Nat Struct Mol Biol. 2005 Mar;12(3):270-3. Epub 2005 Feb 20. PMID:15723078
Page seeded by OCA on Thu Feb 21 15:58:17 2008
