Acetylcholine binding protein

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Template:STRUCTURE 2byn

Acetylcholine binding protein (AChBP) is secreted by snails into cholinergic synapses, where it modulates transmission by binding acetylcholine (ACh). Sequence alignment revealed high similarity to the extracellular domains of the ligand-binding subunits of the nicotinic acetylcholine receptor (nAChR). The crystal structure of the AChBP homopentamer indeed provides a valuable model for identifying the nature of the ligand-binding domains and of the subunit interfaces of the nAChR. Furthermore, crystal structures of complexes of AChBP with various agonists and antagonists have provided detailed insight into the neurotransmitter binding site of nAChRs. The images on the left and right correspond to one representative AChBP structure, i.e. the Acetylcholine binding protein from Aplysia californica (2byn).

3D Structures of Acetylcholine binding protein

Updated on 06-March-2013

AChBP - Apo proteins

2w8e, 2byn, 2y7y - Aplysia californica (AcAChBP)
2xz6, 3sh1, 3sio, 3t4m – AcAChBP (mutant)
1ux2, 1i9b - Lymnaea stagnalis (LsAChBP)
2bj0 - Bulinus truncatus

AChBP + marine biotoxins

2wzy - AcAChBP + 13-desmethyl spirolide C
2x00 - AcAChBP + gymnodimine A

AChBP + partial agonists; Activate the receptor with partial efficacy

2wn9 - AcAChBP + 4-OH-DMXBA
2wnc - AcAChBP + tropisetron
2wnj - AcAChBP + DMXBA
2wnl - AcAChBP + anabaseine

AChBP+ions; Mimicking the ion conductance of the receptor

3gua - AcAChBP + sulfate

AChBP+ neonicotinoids; Insecticides with lower affinity to mammals

3c79 - AcAChBP + imidacloprid
2zju - LsAChBP + imidacloprid
3c84 - AcAChBP + thiacloprid
2zjv - LsAChBP+clothianidin

AChBP+ non-competitive inhibitors which bind at site different than the active site and reduce the rate of receptor reaction

2pjz - AcAChBP+cocaine
2ph9 - AcAChBP+galanthamine
2w8f, 2w8g - AcAChBP + in silico compounds

AChBP+ peptide inhibitors

2c9t, 2byp, 2br8 - AcAChBP+conotoxin IMI
2uz6 – AcAChBP fragment + α-conotoxin
1yi5 - AcAChBP+cobratoxin

AChBP+ nicotinic agonists enhance the action of the nAChR

2pgz - AcAChBP+cocaine
2byr - AcAChBP+methyllycaconitine
2bys - AcAChBP+lobeline
2xnt, 2xnu, 2xnv – AcAChBP + ligand
3peo - AcAChBP+metocurine
3pmz - AcAChBP+tubocurarine
2byq - AcAChBP+epibatidine

AChBP+ ligand homologs of the ligand acetylcholine

1uv6 - LsAChBP+ carbamylcholine
1uw6 - LsAChBP+nicotine
2xz5 – AcAChBP (mutant) + acetylcholine

AChBP+others

2br7 - AcAChBP+ HEPES
2y54, 2y56, 2y57, 2y58 – AcAChBP residues 20-236 + azabicyclo-octan benzoate derivative

AChBP α-7

3sq6, 3sq9 – AchBP h/Ls chimera

Additional Resources

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