Derivation of Triose Phosphate Isomerase

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Derivation

As mentioned above, 1HTI comes from Homo sapiens; the functional annotation is given at the end of this page. The followings are some homologies according to the BLAST results(http://www.uniprot.org/blast/). The configurations are:

<1>Matrix: blosum62 <2>Threshold: 10 <3>Filtered: false <4>Gapped: true <5>Maximum number of hits reported: 250 <6>Database: uniprotkb (Protein) generated for BLAST on Nov 2, 2010

 Accession   Entry_Name     Organism                     GO IDs and terms
 D3DUS9      D3DUS9_HUMAN   Homo sapiens                 0005975  carbohydrate metabolic process
                                                         0006006  glucose metabolic process
                                                         0003824  catalytic activity
                                                         0004807  triose-phosphate isomerase activity
                                                         0005625  soluble fraction
                                                         0005634  nucleus  
 P60175      TPIS_PANTR     Pan troglodytes              0006006  glucose metabolic process
                                                         0006094  gluconeogenesis
                                                         0003824  catalytic activity
                                                         0004807  triose-phosphate isomerase activity  
 P60174      TPIS_HUMAN     Homo sapiens                 0005975  carbohydrate metabolic process
                                                         0006006  glucose metabolic process
                                                         0003824  catalytic activity
                                                         0004807  triose-phosphate isomerase activity  
                                                         0005625  soluble fraction
                                                         0005634  nucleus  
 P00939      TPIS_RABIT     Oryctolagus cuniculus(Rabbit)0006006  glucose metabolic process
                                                         0006094  gluconeogenesis
                                                         0003824  catalytic activity                 
                                                         0004807  triose-phosphate isomerase activity  
 P15426      TPIS_MACMU     Macaca mulatta               0006094  gluconeogenesis
                                                         0006096  glycolysis  
                                                         0003824  catalytic activity
                                                         0004807  triose-phosphate isomerase activity  
 ... ...

What's New~

"Triosephosphate isomerase (TIM) is a perfectly evolved enzyme...Researchers continue to use TIM as a study object for their research, both for computational enzyme mechanism studies as well as for experimental studies."

--R. K. Wierenga, E. G. Kapetaniou and R. Venkatesan, "Triosephosphate isomerase: a highly evolved biocatalyst", Cell Mol Life Sci. 2010 Dec

 "In the present study, we theoretically assessed the effects of 20 point mutations detected previously in a region of the triose-phosphate 
 isomerase gene (tpi) of the protozoan Giardia duodenalis on the three-dimensional structure of the ‘wild-type’ protein (TPI)...the findings 
 provide support for the “neutral theory”, which contends that evolution at the molecular level is not solely shaped by “Darwinian selection
 but also by random drift of selectively neutral or nearly neutral mutants”."

--Nolan MJ, Hofmann A, Jex AR, Gasser RB, "A theoretical study to establish the relationship between the three-dimensional structure of triose-phosphate isomerase of Giardia duodenalis and point mutations in the respective gene", Mol Cell Probes. 2010 Oct

GO Functional Annotation

as TRIOSEPHOSPHATE ISOMERASE(reference: http://www.pdb.org/pdb/explore/explore.do?structureId=1HTI)

(1)Cellular component:

 GO_ID         GO_term
0005625       soluble fraction
0005634       nucleus  
0005829         cytosol  

(2)Biological process:

 GO_ID         GO_term
0008152       metabolic process  
0007275       multicellular organismal development  
0009790       embryonic development  
0005975       carbohydrate metabolic process  
0008610         lipid biosynthetic process  
0019682           glyceraldehyde-3-phosphate metabolic process  
0006633             fatty acid biosynthetic process  
0006006               glucose metabolic process  
0006094                 gluconeogenesis  
0006096           glycolysis  
0006098           pentose-phosphate shunt   

(3)Biochemical function:

 GO_ID         GO_term
0003824       catalytic activity  
0005515       protein binding  
0016853       isomerase activity  
0004807         triose-phosphate isomerase activity

(Created by Mengfei Cao)