Derivation of Triose Phosphate Isomerase

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Derivation

As mentioned above, 1HTI comes from Homo sapiens; the functional annotation is given at the end of this page. The followings are some homologies according to the BLAST results(http://www.uniprot.org/blast/). The configurations are:

<1>Matrix: blosum62 <2>Threshold: 10 <3>Filtered: false <4>Gapped: true <5>Maximum number of hits reported: 250 <6>Database: uniprotkb (Protein) generated for BLAST on Nov 2, 2010

Accession Entry_Name Organism GO IDs and terms D3DUS9 D3DUS9_HUMAN Homo sapiens 0005975 carbohydrate metabolic process

                                                         0006006  glucose metabolic process
                                                         0003824  catalytic activity
                                                         0004807  triose-phosphate isomerase activity
                                                         0005625  soluble fraction
                                                         0005634  nucleus  

P60175 TPIS_PANTR Pan troglodytes 0006006 glucose metabolic process

                                                         0006094  gluconeogenesis
                                                         0003824  catalytic activity
                                                         0004807  triose-phosphate isomerase activity  

P60174 TPIS_HUMAN Homo sapiens 0005975 carbohydrate metabolic process

                                                         0006006  glucose metabolic process
                                                         0003824  catalytic activity
                                                         0004807  triose-phosphate isomerase activity  
                                                         0005625  soluble fraction
                                                         0005634  nucleus  

P00939 TPIS_RABIT Oryctolagus cuniculus(Rabbit)0006006 glucose metabolic process

                                                         0006094  gluconeogenesis
                                                         0003824  catalytic activity                 
                                                         0004807  triose-phosphate isomerase activity  

P15426 TPIS_MACMU Macaca mulatta 0006094 gluconeogenesis

                                                         0006096  glycolysis  
                                                         0003824  catalytic activity
                                                         0004807  triose-phosphate isomerase activity  
 ... ...

What's New~

"Triosephosphate isomerase (TIM) is a perfectly evolved enzyme...Researchers continue to use TIM as a study object for their research, both for computational enzyme mechanism studies as well as for experimental studies."

--R. K. Wierenga, E. G. Kapetaniou and R. Venkatesan, "Triosephosphate isomerase: a highly evolved biocatalyst", Cell Mol Life Sci. 2010 Dec

"In the present study, we theoretically assessed the effects of 20 point mutations detected previously in a region of the triose-phosphate isomerase gene (tpi) of the protozoan Giardia duodenalis on the three-dimensional structure of the ‘wild-type’ protein (TPI)...the findings provide support for the “neutral theory”, which contends that evolution at the molecular level is not solely shaped by “Darwinian selection but also by random drift of selectively neutral or nearly neutral mutants”."

--Nolan MJ, Hofmann A, Jex AR, Gasser RB, "A theoretical study to establish the relationship between the three-dimensional structure of triose-phosphate isomerase of Giardia duodenalis and point mutations in the respective gene", Mol Cell Probes. 2010 Oct

GO Functional Annotation

as TRIOSEPHOSPHATE ISOMERASE(reference: http://www.pdb.org/pdb/explore/explore.do?structureId=1HTI)

(1)Cellular component: GO_ID GO_term 0005625 soluble fraction 0005634 nucleus 0005829 cytosol

(2)Biological process: GO_ID GO_term 0008152 metabolic process 0007275 multicellular organismal development 0009790 embryonic development 0005975 carbohydrate metabolic process 0008610 lipid biosynthetic process 0019682 glyceraldehyde-3-phosphate metabolic process 0006633 fatty acid biosynthetic process 0006006 glucose metabolic process 0006094 gluconeogenesis 0006096 glycolysis 0006098 pentose-phosphate shunt

(3)Biochemical function: GO_ID GO_term 0003824 catalytic activity 0005515 protein binding 0016853 isomerase activity 0004807 triose-phosphate isomerase activity (Created by Mengfei Cao)