2e50

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Template:STRUCTURE 2e50

Contents 1 Crystal structure of SET/TAF-1beta/INHAT 2 Disease 3 Function 4 About this Structure 5 Reference

Crystal structure of SET/TAF-1beta/INHAT

Template:ABSTRACT PUBMED 17360516

Disease

[SET_HUMAN] Note=A chromosomal aberration involving SET is found in some cases of acute undifferentiated leukemia (AUL). Translocation t(6;9)(q21;q34.1) with NUP214/CAN.

Function

[SET_HUMAN] Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone chaperoning. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher.

About this Structure

2e50 is a 4 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

• Muto S, Senda M, Akai Y, Sato L, Suzuki T, Nagai R, Senda T, Horikoshi M. Relationship between the structure of SET/TAF-Ibeta/INHAT and its histone chaperone activity. Proc Natl Acad Sci U S A. 2007 Mar 13;104(11):4285-90. Epub 2007 Mar 6. PMID:17360516
• Muto S, Senda M, Adachi N, Suzuki T, Nagai R, Senda T, Horikoshi M. Purification, crystallization and preliminary X-ray diffraction analysis of human oncoprotein SET/TAF-1beta. Acta Crystallogr D Biol Crystallogr. 2004 Apr;60(Pt 4):712-4. Epub 2004, Mar 23. PMID:15039562 doi:10.1107/S0907444904001647