1oi6
From Proteopedia
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STRUCTURE DETERMINATION OF THE TMP-COMPLEX OF EVAD
Overview
Vancomycin, the last line of defense antibiotic, depends upon the, attachment of the carbohydrate vancosamine to an aglycone skeleton for, antibacterial activity. Vancomycin is a naturally occurring secondary, metabolite that can be produced by bacterial fermentation. To combat, emerging resistance, it has been proposed to genetically engineer bacteria, to produce analogues of vancomycin. This requires a detailed understanding, of the biochemical steps in the synthesis of vancomycin. Here we report, the 1.4 A structure and biochemical characterization of EvaD, an RmlC-like, protein that is required for the C-5' epimerization during synthesis of, dTDP-epivancosamine. EvaD, although clearly belonging to the RmlC class of, enzymes, displays very low activity in the archetypal RmlC reaction, ... [(full description)]
About this Structure
1OI6 is a [Single protein] structure of sequence from [Amycolatopsis orientalis] with TMP and GOL as [ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
The position of a key tyrosine in dTDP-4-Keto-6-deoxy-D-glucose-5-epimerase (EvaD) alters the substrate profile for this RmlC-like enzyme., Merkel AB, Major LL, Errey JC, Burkart MD, Field RA, Walsh CT, Naismith JH, J Biol Chem. 2004 Jul 30;279(31):32684-91. Epub 2004 May 24. PMID:15159413
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