1oj7
From Proteopedia
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STRUCTURAL GENOMICS, UNKNOWN FUNCTION CRYSTAL STRUCTURE OF E. COLI K-12 YQHD
Overview
In the course of a structural genomics program aiming at solving the, structures of Escherichia coli open reading frame (ORF) products of, unknown function, we have determined the structure of YqhD at 2.0A, resolution using the single wavelength anomalous diffraction method at the, Pt edge. The crystal structure of YqhD reveals that it is an, NADP-dependent dehydrogenase, a result confirmed by activity measurements, with several alcohols. The current interpretation of our findings is that, YqhD is an alcohol dehydrogenase (ADH) with preference for alcohols longer, than C(3). YqhD is a dimer of 2x387 residues, each monomer being composed, of two domains, a Rossmann-type fold and an alpha-helical domain. The, crystals contain two dimers in the asymmetric unit. While one of the, dimers ... [(full description)]
About this Structure
1OJ7 is a [Single protein] structure of sequence from [Escherichia coli] with ZN, CL, NZQ and BO3 as [ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Crystal structure of E.coli alcohol dehydrogenase YqhD: evidence of a covalently modified NADP coenzyme., Sulzenbacher G, Alvarez K, Van Den Heuvel RH, Versluis C, Spinelli S, Campanacci V, Valencia C, Cambillau C, Eklund H, Tegoni M, J Mol Biol. 2004 Sep 10;342(2):489-502. PMID:15327949
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