3k3r

From Proteopedia

proteopedia linkproteopedia link

Template:STRUCTURE 3k3r

Contents 1 Unrefined crystal structure of a LexA-DNA complex 2 Function 3 About this Structure 4 Reference

Unrefined crystal structure of a LexA-DNA complex

Template:ABSTRACT PUBMED 020703307

Function

[LEXA_ECOLI] Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. Binds to the 16 bp palindromic sequence 5'-CTGTATATATATACAG-3'. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair.^{[1] [2]}

About this Structure

3k3r is a 4 chain structure with sequence from Escherichia coli k-12. Full crystallographic information is available from OCA.

Reference

• Zhang AP, Pigli YZ, Rice PA. Structure of the LexA-DNA complex and implications for SOS box measurement. Nature. 2010 Aug 12;466(7308):883-6. PMID:20703307 doi:10.1038/nature09200

1. ↑ Little JW, Mount DW, Yanisch-Perron CR. Purified lexA protein is a repressor of the recA and lexA genes. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4199-203. PMID:7027255
2. ↑ Brent R, Ptashne M. Mechanism of action of the lexA gene product. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4204-8. PMID:7027256