This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
3ks4
From Proteopedia
Contents |
Crystal structure of Reston ebolavirus VP35 RNA binding domain
Template:ABSTRACT PUBMED 20018665
Function
[VP35_EBORR] Acts as a polymerase cofactor in the RNA polymerase transcription and replication complex. Prevents establishment of cellular antiviral state by blocking virus-induced phosphorylation and activation of interferon regulatory factor 3 (IRF3), a transcription factor critical for the induction of interferons alpha and beta. The mechanism by which this blockage occurs remains incompletely defined, a hypothesis suggests that VP35 dsRNA-binding activity prevents activation of IRF3 by sequestering dsRNA. Also inhibits the antiviral effect mediated by the interferon-induced, double-stranded RNA-activated protein kinase EIF2AK2/PKR (By similarity).
About this Structure
3ks4 is a 2 chain structure with sequence from Reston ebolavirus. Full crystallographic information is available from OCA.
Reference
- Kimberlin CR, Bornholdt ZA, Li S, Woods VL Jr, Macrae IJ, Saphire EO. Ebolavirus VP35 uses a bimodal strategy to bind dsRNA for innate immune suppression. Proc Natl Acad Sci U S A. 2009 Dec 14. PMID:20018665
Categories: Reston ebolavirus | Bornholdt, Z A. | Kimberlin, C R. | Li, S. | Macrae, I J. | Saphire, E O. | Woods, V L. | Ebolavirus | Host cytoplasm | Interferon | Interferon antiviral system evasion | Protein-rna complex | Reston | Rna binding protein | Rna replication | Rna-binding | Rna-binding protein | Transcription | Viral protein | Virion
