3l3q
From Proteopedia
Contents |
Mouse importin alpha-pepTM NLS peptide complex
Template:ABSTRACT PUBMED 20406804
Function
[IMA2_MOUSE] Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
About this Structure
3l3q is a 3 chain structure with sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
- Yang SN, Takeda AA, Fontes MR, Harris JM, Jans DA, Kobe B. Probing the specificity of binding to the major nuclear localization sequence-binding site of importin-alpha using oriented peptide library screening. J Biol Chem. 2010 Jun 25;285(26):19935-46. Epub 2010 Apr 20. PMID:20406804 doi:10.1074/jbc.M109.079574
