3zoa

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Template:STRUCTURE 3zoa

Contents

The structure of Trehalose Synthase (TreS) of Mycobacterium smegmatis in complex with acarbose

Template:ABSTRACT PUBMED 23735230

Function

[TRES_MYCS2] Catalyzes the reversible interconversion of maltose and trehalose by transglucosylation. Maltose is the preferred substrate. To a lesser extent, also displays amylase activity, catalyzing the endohydrolysis of (1->4)-alpha-D-glucosidic linkages in glycogen and maltooligosaccharides such as maltoheptaose, to produce maltose which then can be converted to trehalose. TreS plays a key role in the utilization of trehalose for the production of glycogen, and might also function as a sensor and/or regulator of trehalose levels within the cell. Thus, when trehalose levels in the cell become dangerously low, TreS can expedite the conversion of glycogen to maltose via its amylase activity and then convert the maltose to trehalose; but this enzyme also can expedite or promote the conversion of trehalose to glycogen when cytoplasmic trehalose levels become too high. Is also able to catalyze the hydrolytic cleavage of alpha-aryl glucosides, as well as alpha-glucosyl fluoride in vitro.[1] [2] [3] [4]

About this Structure

3zoa is a 2 chain structure with sequence from Mycobacterium smegmatis. Full crystallographic information is available from OCA.

Reference

  • Caner S, Nguyen N, Aguda A, Zhang R, Pan YT, Withers SG, Brayer GD. The structure of the Mycobacterium smegmatis trehalose synthase reveals an unusual active site configuration and acarbose-binding mode. Glycobiology. 2013 Jul 5. PMID:23735230 doi:10.1093/glycob/cwt044
  1. ↑ Pan YT, Koroth Edavana V, Jourdian WJ, Edmondson R, Carroll JD, Pastuszak I, Elbein AD. Trehalose synthase of Mycobacterium smegmatis: purification, cloning, expression, and properties of the enzyme. Eur J Biochem. 2004 Nov;271(21):4259-69. PMID:15511231 doi:10.1111/j.1432-1033.2004.04365.x
  2. ↑ Pan YT, Carroll JD, Asano N, Pastuszak I, Edavana VK, Elbein AD. Trehalose synthase converts glycogen to trehalose. FEBS J. 2008 Jul;275(13):3408-20. doi: 10.1111/j.1742-4658.2008.06491.x. Epub, 2008 May 23. PMID:18505459 doi:10.1111/j.1742-4658.2008.06491.x
  3. ↑ Elbein AD, Pastuszak I, Tackett AJ, Wilson T, Pan YT. The last step in the conversion of trehalose to glycogen:A mycobacterial enzyme that transfers maltose from maltose-1-phosphate to glycogen. J Biol Chem. 2010 Jan 29. PMID:20118231 doi:M109.033944
  4. ↑ Zhang R, Pan YT, He S, Lam M, Brayer GD, Elbein AD, Withers SG. Mechanistic analysis of trehalose synthase from Mycobacterium smegmatis. J Biol Chem. 2011 Oct 14;286(41):35601-9. doi: 10.1074/jbc.M111.280362. Epub 2011, Aug 12. PMID:21840994 doi:10.1074/jbc.M111.280362

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