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2jdg
From Proteopedia
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AFFILIN BASED ON HUMAN GAMMA-B CRYSTALLIN
Overview
The concept of novel binding proteins as an alternative to antibodies has undergone rapid development and is now ready for practical use in a wide range of applications. Alternative binding proteins, based on suitable scaffolds with desirable properties, are selected from combinatorial libraries in vitro. Here, we describe an approach using a beta-sheet of human gamma-B-crystallin to generate a universal binding site through randomization of eight solvent-exposed amino acid residues selected according to structural and sequence analyses. Specific variants, so-called Affilin, have been isolated from a phage display library against a variety of targets that differ considerably in size and structure. The isolated Affilin variants can be produced in Escherichia coli as soluble proteins and have a high level of thermodynamic stability. The crystal structures of the human wild-type gamma-B-crystallin and a selected Affilin variant have been determined to 1.7 A and 2.0 A resolution, respectively. Comparison of the two molecules indicates that the human gamma-B-crystallin tolerates amino acid exchanges with no major structural change. We conclude that the intrinsically stable and easily expressed gamma-B-crystallin provides a suitable framework for the generation of novel binding molecules.
About this Structure
2JDG is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Affilin-novel binding molecules based on human gamma-B-crystallin, an all beta-sheet protein., Ebersbach H, Fiedler E, Scheuermann T, Fiedler M, Stubbs MT, Reimann C, Proetzel G, Rudolph R, Fiedler U, J Mol Biol. 2007 Sep 7;372(1):172-85. Epub 2007 Jun 22. PMID:17628592
Page seeded by OCA on Thu Feb 21 18:02:02 2008
Categories: Homo sapiens | Single protein | Ebersbach, H. | Fiedler, E. | Fiedler, M. | Fiedler, U. | Proetzel, G. | Reimann, C. | Rudolph, R. | Scheuermann, T. | Stubbs, M T. | Affilin | Artificial binding protein | Eye lens protein | Gamma crystallin | Oxidation | Phosphorylation | Polymorphism | Structural protein
