Transcription initiation factors (TFII) are proteins which enable RNA polymerase II to bind to the DNA template in order to start the transcription process. TFIID recognizes the promoter sequence on the DNA while TFIIB bridges between TFIID and RNA polymerase II. TFIIA is one of several TFs which are required for transcription. TFIIA aids in binding the TATA-binding protein (TBP) to the TATA box containing sequence of the DNA. TFIIF binds RNA polymerase III prohibiting it to bind to DNA outside the promoter.
3D structures of transcription initiation factors
Updated on 11-August-2013
TFIIA
1ytf, 1nh2, 1rm1 – yTFIIA + TBP + DNA – yeast
1nvp – hTFIIA + TATA-binding protein + DNA – human
TFIIB
1pft – TFIIB N terminal – Pyrococcus furiosus – NMR
1tfb – hTFIIB - NMR
1dl6 - hTFIIB N terminal – NMR
1rly - hTFIIB Zn ribbon domain + Zn – NMR
1ro4 - hTFIIB Zn ribbon domain – NMR
1vol, 1c9b – hTFIIB + TBP + DNA
3k1f, 3k7a, 4bbr, 4bbs – yTFIIB + RNA polymerase II
1ais, 1d3u - TFIIB C terminal + TBP + DNA – Pyrococcus woesei
TFIID
1bh8 – hTFIID
1eqf – hTFIID subunit 1
3uv5, 3uv4 – hTFIID subunit 1 bromodomain
3aad – hTFIID subunit 1 bromodomain + histone chaperone ASF1A
1tba – TFIID – Drosophila melanogaster - NMR
1bh9 – hTFIID + PCMBS
1qn3, 1qn4, 1qn5, 1qn6, 1qn7, 1qn8, 1qn9, 1qna, 1qnb, 1qnc, 1qne – TFIID + DNA – Arabidopsis thaliana
TFIIE
1vd4 - hTFIIB Zn finger domain – NMR
2rnq – hTFIIE C terminal - NMR
2rnr – hTFIIE C terminal + TFIIH PH domain- NMR
TFIIF
1f3u – hTFIIF
1nha – hTFIIF C terminal - NMR
1i27 – hTFIIF C terminal (mutant)
1j2x – hTFIIF C terminal + RNA polymerase CTD phosphatase peptide
1onv – hTFIIF C terminal + serine phosphatase peptide - NMR
2k7l – hTFIIF C terminal + FCP1 peptide - NMR
